Insertion junction: LMJ.RY0402.107276_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.107276
Chromosome:chromosome 9
Location:6284890
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre09.g406500 (1 of 1) PF07814 - Wings apart-like protein regulation of heterochromatin (WAPL) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCTCGGCACGCGACCTTTCACCCAACATA
Internal bar code:GTTGGTTGCGTCGGGCGCGTGT

Confirmation - LEAP-Seq

LEAP-Seq distance:177
LEAP-Seq percent confirming:67.333
LEAP-Seq n confirming:1179
LEAP-Seq n nonconfirming:572
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:CCTGCTCATCAACCTCACCT
Suggested primer 2:ACACACCCTCCACACACTCA