Insertion junction: LMJ.RY0402.107283_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.107283
Chromosome:chromosome_3
Location:8039390
Confidence (%):58
Locus systematic id Locus common name Defline Orientation Feature
Cre03.g201400 PPP16,PP2C6 Phosphoprotein phosphatase 2C sense 3'UTR

Insertion site details

Flanking sequence (orientation from cassette outwards):GCCGACTGGGTCTGCAGGGGCATCTTGCCC
Internal bar code:GAGGCACCGCAATAACTCTATC

Confirmation - LEAP-Seq

LEAP-Seq distance:145
LEAP-Seq percent confirming:84.6386
LEAP-Seq n confirming:281
LEAP-Seq n nonconfirming:51
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GTACCTGTTCGTGCCCAAGT
Suggested primer 2:GCTGAGTCCCGAGAAACAAG