Insertion junction: LMJ.RY0402.107344_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.107344
Chromosome:chromosome 2
Location:4836554
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g103100 SDR5 (1 of 1) K11165 - dehydrogenase/reductase SDR family member 7 (DHRS7); Short-chain dehydrogenase/reductase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCAAAAACTGTCGCTGTGCCGTGCCCTTG
Internal bar code:TGTTTACGTACGGTTTACCAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:0
LEAP-Seq percent confirming:0.0
LEAP-Seq n confirming:0
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:0

Suggested primers for confirmation by PCR

Suggested primer 1:TAAAGCAAGGCCCTGTCACT
Suggested primer 2:ATCTCGCAGCATCACCTTCT