| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.107355 |
| Chromosome: | chromosome 6 |
| Location: | 8584671 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g308700 | (1 of 1) PTHR31595:SF1 - LONG-CHAIN-ALCOHOL O-FATTY-ACYLTRANSFERASE 1-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGCTTCCTGTCGGGGCTGCTGACCTTCT |
| Internal bar code: | TTTTGCATCTCGGTTAGGGCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 184 |
| LEAP-Seq percent confirming: | 99.403 |
| LEAP-Seq n confirming: | 333 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACAGCAACACATCCACAGC |
| Suggested primer 2: | CATGTACACCACTGGCATCC |