Insertion junction: LMJ.RY0402.107371_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.107371
Chromosome:chromosome 12
Location:7945829
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre12.g552300 CTL3 (1 of 1) IPR001304//IPR016187//IPR024616 - C-type lectin // C-type lectin fold // Pherophorin; Putative pherophorin with C-type lectin CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCTTAAAAACAACACGTGAGGGAGCAAGGC
Internal bar code:TGGTGTTAACATTCTATACAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:317
LEAP-Seq percent confirming:98.8321
LEAP-Seq n confirming:1354
LEAP-Seq n nonconfirming:16
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CCTAGCGCTCACCAGGATAG
Suggested primer 2:GTTCGCAGCCTGGATAAGAG