| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.107392 |
| Chromosome: | chromosome 7 |
| Location: | 838525 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g318551 | CYA8 | Adenylate cyclase; (1 of 1) PF00211//PF13855 - Adenylate and Guanylate cyclase catalytic domain (Guanylate_cyc) // Leucine rich repeat (LRR_8) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTACTTGCAGCTGCACATCAGCCATGTC |
| Internal bar code: | TCCCGAGCGGGCCGCGCTGACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 271 |
| LEAP-Seq percent confirming: | 98.9979 |
| LEAP-Seq n confirming: | 1877 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACGCAACTCACACACACAG |
| Suggested primer 2: | GCACCTCGCTCTTTCAGAAG |