Insertion junction: LMJ.RY0402.107400_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.107400
Chromosome:chromosome 4
Location:3045827
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre04.g225200 FAP311 Flagellar Associated Protein 311; (1 of 5) PF13374 - Tetratricopeptide repeat (TPR_10) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGACTGCGCTTTGCATGTGCAAACACGTTT
Internal bar code:CCTGGTGAGGCCATGCTGGTAA

Confirmation - LEAP-Seq

LEAP-Seq distance:93
LEAP-Seq percent confirming:5.12821
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:37
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:ACACACACACACACACGCAC
Suggested primer 2:CCGTGTGTGTGTGTGTGTGT