Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.107443 |
Chromosome: | chromosome_12 |
Location: | 6478300 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre12.g538300 | LF5,FAP247 | sense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TATCTTGAACAGACATGTGAGGAGTGTGCA |
Internal bar code: | GAAATTGATCGAGTAGGAGGCT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 52 |
LEAP-Seq percent confirming: | 98.5916 |
LEAP-Seq n confirming: | 70 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTTTGGGCAGAACCAGATGT |
Suggested primer 2: | AGCCCCGGCTAGAGAAGTAG |