Insertion junction: LMJ.RY0402.107481_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.107481
Chromosome:chromosome 1
Location:7523137
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g054150 NTRC1,NTR4 (1 of 1) PTHR10438//PTHR22912//PTHR22912:SF48 - THIOREDOXIN // DISULFIDE OXIDOREDUCTASE // NADPH-DEPENDENT THIOREDOXIN REDUCTASE 3; NADPH-dependent thioredoxin reductase C, chloroplastic 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATGCATCCCGACCCGAACCTCGTCACCAAA
Internal bar code:CCATAGCGGTAGGAGGACGACG

Confirmation - LEAP-Seq

LEAP-Seq distance:628
LEAP-Seq percent confirming:99.6931
LEAP-Seq n confirming:1624
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:TTTCCCGATGCTCTCTCAGT
Suggested primer 2:GATGAGTACACGGGCAAGGT