| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.107493 |
| Chromosome: | chromosome_8 |
| Location: | 307912 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre08.g358564 | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GCCATCCAGTTGCCTGACCCCTCCCCTCCC |
| Internal bar code: | ACACAGAGACGTTGCGTCGCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 89 |
| LEAP-Seq percent confirming: | 97.2222 |
| LEAP-Seq n confirming: | 70 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCAGGAGTGCTGACCTTGT |
| Suggested primer 2: | GAAGTTAGGGACGGACACGA |