| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.107515 |
| Chromosome: | chromosome 3 |
| Location: | 753361 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g146227 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAGGCTCCTTGGAGCGCGCCATGGCTAAA |
| Internal bar code: | GCAGTTCAGTCTCGCCAAAACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 695 |
| LEAP-Seq percent confirming: | 97.8846 |
| LEAP-Seq n confirming: | 1018 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCATGTCTGGGCTCCCTATC |
| Suggested primer 2: | TGATGCGTGAAGACAGGAAG |