| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.107519 |
| Chromosome: | chromosome 2 |
| Location: | 5976336 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g112150 | (1 of 10) 3.1.1.5 - Lysophospholipase / Phospholipase B | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGATCCACATCCGCGACCTTGATGATGGCG |
| Internal bar code: | TGTGCTGGGCAGACGTTCAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 339 |
| LEAP-Seq percent confirming: | 88.4261 |
| LEAP-Seq n confirming: | 10849 |
| LEAP-Seq n nonconfirming: | 1420 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAGATGCCGTTGGTGGTCT |
| Suggested primer 2: | CATGAGCTGTGAGTGGCTGT |