| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.107546 |
| Chromosome: | chromosome 11 |
| Location: | 2990352 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g478400 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCTCCTGCAGCAGTCCTGCACCCCTAGC |
| Internal bar code: | AGTCGCAATTCGGTGCGTCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 462 |
| LEAP-Seq percent confirming: | 99.6553 |
| LEAP-Seq n confirming: | 29204 |
| LEAP-Seq n nonconfirming: | 101 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGCACTACGACTGGCAATG |
| Suggested primer 2: | CATGTGTCCAACCCTCTCCT |