| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.107562 |
| Chromosome: | chromosome 13 |
| Location: | 2546033 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g580700 | DAL2 | allantoate deiminase; (1 of 1) 3.5.3.9 - Allantoate deiminase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTCTGTGAGCGTGTGTTACGTATTGTGCG |
| Internal bar code: | GCTCGGTTCGCAGCTTCGGCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 97 |
| LEAP-Seq percent confirming: | 99.8226 |
| LEAP-Seq n confirming: | 2251 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACACGAGATAGGGGACACG |
| Suggested primer 2: | ATCAAAACAAGGTGGGCAAG |