Insertion junction: LMJ.RY0402.107568_3

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.107568
Chromosome:chromosome 16
Location:4031191
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre16.g670200 Microtubule-associated protein cysteine-rich PDZ-binding protein; (1 of 2) PTHR11805:SF1 - CYSTEINE-RICH PDZ-BINDING PROTEIN intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTACCCAATGCCTGTGTCCCCATGTCGAC
Internal bar code:TGTGACGGGCGCTAACCGGACA

Confirmation - LEAP-Seq

LEAP-Seq distance:371
LEAP-Seq percent confirming:94.8364
LEAP-Seq n confirming:4059
LEAP-Seq n nonconfirming:221
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:CTGTTTGCACGTCTTGCATT
Suggested primer 2:TTGGGGAAACAATTGTGGAT