| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.107585 |
| Chromosome: | chromosome 7 |
| Location: | 1839997 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g325732 | OPR109 | OctotricoPeptide Repeat protein 109 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCTGTTTGCCGGTTGCGCTCTCACTTCGC |
| Internal bar code: | GCGGGGCGAGAAACAATACGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 616 |
| LEAP-Seq percent confirming: | 99.8596 |
| LEAP-Seq n confirming: | 1422 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTATTCATTGCCCCAGTCT |
| Suggested primer 2: | TGCTGATCGAAAAGTTCGTG |