| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.107587 |
| Chromosome: | chromosome 10 |
| Location: | 1075336 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g425350 | (1 of 6) IPR001245//IPR011009 - Serine-threonine/tyrosine-protein kinase catalytic domain // Protein kinase-like domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCCCCTCCTCCACGCCTGGACTTTGCGGA |
| Internal bar code: | ACGCGTGGTCTGAGGTTCGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 547 |
| LEAP-Seq percent confirming: | 99.3435 |
| LEAP-Seq n confirming: | 2724 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCAGTAGAATGCATGCCGA |
| Suggested primer 2: | ACTGTTAGCAACCAGGCACC |