| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.107635 |
| Chromosome: | chromosome 12 |
| Location: | 7657875 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g554450 | 3'UTR|CDS|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGTGCCGACTCGTCCGCGCGCGATGCTT |
| Internal bar code: | TTCAGAGCACCTGAGTGATGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 411 |
| LEAP-Seq percent confirming: | 98.7355 |
| LEAP-Seq n confirming: | 8199 |
| LEAP-Seq n nonconfirming: | 105 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATGGGCGTAAGTTGTTTGC |
| Suggested primer 2: | CCACGCAACACACAGTTCTT |