Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.107669 |
Chromosome: | chromosome 9 |
Location: | 275125 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
intergenic |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACACACACACACCGGGCGAAGGGAGGGAGG |
Internal bar code: | GGGCAAGCAGCCGACGAGAAC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 253 |
LEAP-Seq percent confirming: | 99.2833 |
LEAP-Seq n confirming: | 2355 |
LEAP-Seq n nonconfirming: | 17 |
LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCCGTACACACACACACACA |
Suggested primer 2: | COULD_NOT_FIND_PRIMER |