| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.107671 |
| Chromosome: | chromosome 10 |
| Location: | 3940536 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g448350 | (1 of 3) PTHR10361 - SODIUM-BILE ACID COTRANSPORTER | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATGCTCACCTAGCCCCAGGGGCGTGCCGA |
| Internal bar code: | CCATGGACGGAGATATGATGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 586 |
| LEAP-Seq percent confirming: | 99.3662 |
| LEAP-Seq n confirming: | 1411 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCCACCCATTCACTCAATC |
| Suggested primer 2: | AGCGATGCCTAAGCTGGTAA |