| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.107674 |
| Chromosome: | chromosome 2 |
| Location: | 1866191 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g087450 | (1 of 4) PTHR16255:SF4 - SPORULATION PROTEIN RMD8 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCCCTCCGGCACTCGGCCCCGCCCCGGC |
| Internal bar code: | TGAGGATCTCCGGGGGTTGTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 885 |
| LEAP-Seq percent confirming: | 22.2222 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGGTCTCAAGCAGAAAGG |
| Suggested primer 2: | CCCAAGGTCCTTTCCTAAGC |