| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.107734 |
| Chromosome: | chromosome 10 |
| Location: | 5792041 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g461400 | (1 of 1) PTHR22957:SF212 - [PROTEIN ADP-RIBOSYLARGININE] HYDROLASE | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGTGCTATTTATTCTTTTTGATTAGCTG |
| Internal bar code: | GGTGCAACGGGAGAAAGGCTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 628 |
| LEAP-Seq percent confirming: | 99.3773 |
| LEAP-Seq n confirming: | 2713 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAGGCTGAGGCTGTGTATG |
| Suggested primer 2: | CTAGGCGCAATCTCAACACA |