Insertion junction: LMJ.RY0402.107740_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.107740
Chromosome:chromosome 13
Location:1864726
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre13.g575500 (1 of 726) IPR011009 - Protein kinase-like domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGCGTCCCCCGAGTCCTGGTGGTCGCAAA
Internal bar code:CTGCCGTGACTCACTAGTTAAA

Confirmation - LEAP-Seq

LEAP-Seq distance:461
LEAP-Seq percent confirming:42.2478
LEAP-Seq n confirming:624
LEAP-Seq n nonconfirming:853
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GTCCGAAGTAAGCTCATCGC
Suggested primer 2:CTTTAGGACGACTTGCAGCC