| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.107760 |
| Chromosome: | chromosome 1 |
| Location: | 2795508 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g016556 | (1 of 1) K03115 - casein kinase II subunit beta (CSNK2B) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAAGTTGCCTGCCTTAGCGAGCTCGGTGA |
| Internal bar code: | GGTCCCCCTGTATTATCAAATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 545 |
| LEAP-Seq percent confirming: | 99.5984 |
| LEAP-Seq n confirming: | 7193 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCTGAACAGGCAGGATGTC |
| Suggested primer 2: | TGTACGTGGTGTTGAGGGAA |