| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.107778 |
| Chromosome: | chromosome_7 |
| Location: | 4199227 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre07.g342200 | IFT70,FAP259,CrDYF-1 | Flagellar Associated Protein | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CCCCCTCGCACAGCCCATCCACCCTCCCTT |
| Internal bar code: | GTAAGTTGTGGGGGCCGTGGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 369 |
| LEAP-Seq percent confirming: | 37.6812 |
| LEAP-Seq n confirming: | 26 |
| LEAP-Seq n nonconfirming: | 43 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCTGCGATACGGAGGTCTA |
| Suggested primer 2: | AGAGCGTGGCCTATGCTTTA |