| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.107809 |
| Chromosome: | chromosome 2 |
| Location: | 4828740 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g103000 | FKM3 | DNA methylase in the FkbM family; (1 of 13) PF05050 - Methyltransferase FkbM domain (Methyltransf_21) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTCCGTGGCTGCTTCGCTGCGCAGACACC |
| Internal bar code: | CTTTCGAAAGCGACCACCGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 739 |
| LEAP-Seq percent confirming: | 99.8512 |
| LEAP-Seq n confirming: | 3356 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGTACTTGCCACAGCCAG |
| Suggested primer 2: | GGGGCAAGATATTGAGGTGA |