Insertion junction: LMJ.RY0402.107815_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.107815
Chromosome:chromosome 1
Location:1403873
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g007000 (1 of 4) 3.6.3.29 - Molybdate-transporting ATPase; ABC transporter, half intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCCACACACCTTGCTCGTATTGGTGAGGT
Internal bar code:TTCATCCTCCGACGTCCTTGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:185
LEAP-Seq percent confirming:99.9037
LEAP-Seq n confirming:2075
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:TTGGGGTAGTCAGACGGTTC
Suggested primer 2:TCAAGTGCGTATCGTGCTTC