| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.107844 |
| Chromosome: | chromosome 5 |
| Location: | 1382743 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g244300 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAAGGAGGAGCTACGGAAGAAGGCGGAGG |
| Internal bar code: | GCCCCAAGTTTGTATCACTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 633 |
| LEAP-Seq percent confirming: | 90.9396 |
| LEAP-Seq n confirming: | 1355 |
| LEAP-Seq n nonconfirming: | 135 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACAGCACATACCATCCTG |
| Suggested primer 2: | CACTACTTTTCTCAGCGCCC |