Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.107864 |
Chromosome: | chromosome_7 |
Location: | 2057225 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre07.g325762 | DRC11 | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GGCTGCGGGGTGCGGGGAGGGCCCCGAACA |
Internal bar code: | GTCAGTATTGTCGTAAAGGTGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 271 |
LEAP-Seq percent confirming: | 97.8661 |
LEAP-Seq n confirming: | 1330 |
LEAP-Seq n nonconfirming: | 29 |
LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCATACCGTATTGTGTGCCA |
Suggested primer 2: | GCTCACTTCTTCTTGCCACC |