Insertion junction: LMJ.RY0402.107877_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.107877
Chromosome:chromosome 3
Location:8532838
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g201552 MMP8 Metalloproteinase of VMP family; (1 of 1) IPR003882//IPR008752//IPR013320 - Pistil-specific extensin-like protein // Peptidase M11, gametolysin // Concanavalin A-like lectin/glucanase domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AATTCATATTTGGCCAACAGCAACGGCCAC
Internal bar code:GGGGTGTCGAATCCCTCAGTCC

Confirmation - LEAP-Seq

LEAP-Seq distance:940
LEAP-Seq percent confirming:99.4555
LEAP-Seq n confirming:548
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:AAGGACAAGAACGGCAAAGA
Suggested primer 2:CTGGAGAAGCCGTGGTAGAG