| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.107884 |
| Chromosome: | chromosome 4 |
| Location: | 2745639 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g223550 | (1 of 26) IPR011990 - Tetratricopeptide-like helical domain | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCATAGCGCCCGAGGCGCCCGTGTGTTG |
| Internal bar code: | AGTATTGAGTAGACTTTGGAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1481 |
| LEAP-Seq percent confirming: | 99.6233 |
| LEAP-Seq n confirming: | 5025 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTTGTTTGGCTGCCGTATT |
| Suggested primer 2: | GGAGTAGGTATGGATGCGGA |