Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.107893 |
Chromosome: | chromosome_10 |
Location: | 2301805 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre10.g434600 | FAP148 | Flagellar Associated Protein | sense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GTGGTACTGTCAACGAGACACCTGCTGCGG |
Internal bar code: | AGGGGTCGTTTTGTTTATAGGA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 661 |
LEAP-Seq percent confirming: | 98.8839 |
LEAP-Seq n confirming: | 1772 |
LEAP-Seq n nonconfirming: | 20 |
LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AGATGCTCATGTGAGTTGCG |
Suggested primer 2: | TTAACCCGATTCCAAACTGC |