| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.107918 |
| Chromosome: | chromosome 6 |
| Location: | 2694592 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g270350 | AMYB2,AMB2 | Beta-amylase 2; (1 of 3) 3.2.1.2 - Beta-amylase / Saccharogen amylase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGAAACTGCACAGCTATCCCCCCAACCAT |
| Internal bar code: | ACACCGAATAAAGCGCGCTGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 934 |
| LEAP-Seq percent confirming: | 50.0 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTATTCAAAGTGCACGGGA |
| Suggested primer 2: | AACAATCCTCTCGCCTGCTA |