| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.107919 |
| Chromosome: | chromosome 2 |
| Location: | 739345 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g078500 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGACACATGATGCAGTCAGACATGACAATA |
| Internal bar code: | CGTAGGCAGGGCTTGAGGGCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 611 |
| LEAP-Seq percent confirming: | 98.9453 |
| LEAP-Seq n confirming: | 1501 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCAACTCCAATCAGGTTGT |
| Suggested primer 2: | AATTGGAATGAAACCCCCTC |