Insertion junction: LMJ.RY0402.107919_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.107919
Chromosome:chromosome 2
Location:2718349
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g093600 TEH3 (1 of 6) PF03061 - Thioesterase superfamily (4HBT); Thioesterase-like protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCAGTCCATTGCGTGCCATGAACTTGAAC
Internal bar code:CTCTGGAGTCGACACGGATCCG

Confirmation - LEAP-Seq

LEAP-Seq distance:295
LEAP-Seq percent confirming:97.0606
LEAP-Seq n confirming:1585
LEAP-Seq n nonconfirming:48
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:TAAAGGCACGGGTAAAGTGG
Suggested primer 2:GTGTGTTGCCCATGTCACTC