| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.107943 |
| Chromosome: | chromosome 2 |
| Location: | 4233385 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g098750 | (1 of 7) PF00027//PF00520 - Cyclic nucleotide-binding domain (cNMP_binding) // Ion transport protein (Ion_trans) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGACGACGACGAGCTGATGTACGGCAATGA |
| Internal bar code: | CGAGCCGGATGACTGTCGTTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 24 |
| LEAP-Seq percent confirming: | 95.288 |
| LEAP-Seq n confirming: | 182 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGAGTGCGTTGCTTATGCT |
| Suggested primer 2: | CGTTACCGGAGAGATCTTGG |