| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.108015 |
| Chromosome: | chromosome 16 |
| Location: | 7316641 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g684379 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTCAAAGGGAGGGGCAGGGACGACTTGTG |
| Internal bar code: | AAAGGTGGGGCTGATTGCCTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 630 |
| LEAP-Seq percent confirming: | 94.1092 |
| LEAP-Seq n confirming: | 655 |
| LEAP-Seq n nonconfirming: | 41 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATGCCAAGTACAGACGCAA |
| Suggested primer 2: | CTTGCCAAGGGTTTTGTTGT |