| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.108025 |
| Chromosome: | chromosome 12 |
| Location: | 4829760 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g524700 | HAD4 | Haloacid dehalogenase-like hydrolase; (1 of 1) K18551 - pyrimidine and pyridine-specific 5'-nucleotidase (SDT1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGAAGCAGTCGCTGAGGCCCATGCGCGCC |
| Internal bar code: | CCACTCTGTTGCAGACTCAGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 363 |
| LEAP-Seq percent confirming: | 97.1774 |
| LEAP-Seq n confirming: | 241 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTACTCTGGCCTGCCTTTG |
| Suggested primer 2: | AAGCCCCAACTATGTGCAAC |