| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.108035 |
| Chromosome: | chromosome 13 |
| Location: | 3667478 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g588850 | (1 of 3) PF10250 - GDP-fucose protein O-fucosyltransferase (O-FucT) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGTATTGGTAATGCGTCCCACACAATCAG |
| Internal bar code: | TGGAATCAATACACTGCAAACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 575 |
| LEAP-Seq percent confirming: | 99.1111 |
| LEAP-Seq n confirming: | 1115 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATGTTCGTCGCCTTGTACC |
| Suggested primer 2: | CGCACGACTTAGCTGTGGTA |