Insertion junction: LMJ.RY0402.108037_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.108037
Chromosome:chromosome 11
Location:2921202
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre11.g478025 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGAATAAGTCTGCCGGCATTGACGGTGCG
Internal bar code:ACCAAATCAGCTTAGATGATAA

Confirmation - LEAP-Seq

LEAP-Seq distance:630
LEAP-Seq percent confirming:94.4294
LEAP-Seq n confirming:13951
LEAP-Seq n nonconfirming:823
LEAP-Seq n unique pos:72

Suggested primers for confirmation by PCR

Suggested primer 1:GCCTGTTTCCAACACCACTT
Suggested primer 2:CGCACGTGACAAGGTTTAGA