Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.108037 |
Chromosome: | chromosome 11 |
Location: | 2921202 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre11.g478025 | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGAATAAGTCTGCCGGCATTGACGGTGCG |
Internal bar code: | ACCAAATCAGCTTAGATGATAA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 630 |
LEAP-Seq percent confirming: | 94.4294 |
LEAP-Seq n confirming: | 13951 |
LEAP-Seq n nonconfirming: | 823 |
LEAP-Seq n unique pos: | 72 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCCTGTTTCCAACACCACTT |
Suggested primer 2: | CGCACGTGACAAGGTTTAGA |