| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.108046 |
| Chromosome: | chromosome 12 |
| Location: | 2214825 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g508250 | (1 of 1) PTHR22975:SF9 - F6D8.33-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTTTGCAAGTGTGTTATATCGTGCCTGA |
| Internal bar code: | GTGGTAGTACGGAGTTATAGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 676 |
| LEAP-Seq percent confirming: | 99.6149 |
| LEAP-Seq n confirming: | 21213 |
| LEAP-Seq n nonconfirming: | 82 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTAAGCATTTTCAGGCGG |
| Suggested primer 2: | CCACGCCATCTAAACCTTGT |