| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.108078 |
| Chromosome: | chromosome 7 |
| Location: | 2320412 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g327950 | MAW9 | Membrane-associated hydroxyproline-rich glycoprotein 9 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGACCAGCCAATCCACACGGAGGCGTTGGA |
| Internal bar code: | TCCGTGTCATATACTCATGCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 237 |
| LEAP-Seq percent confirming: | 99.3639 |
| LEAP-Seq n confirming: | 781 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCCTGGATTACCCTCCCTC |
| Suggested primer 2: | TCATCACGTAGGAGCAGACG |