Insertion junction: LMJ.RY0402.108116_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.108116
Chromosome:chromosome 6
Location:2660372
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g270050 (1 of 587) 2.7.11.1 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCAGCGCGCAAGAGCACCATGCCCGCCCA
Internal bar code:GCCAGCTACGAATGATACTTGA

Confirmation - LEAP-Seq

LEAP-Seq distance:60
LEAP-Seq percent confirming:30.3303
LEAP-Seq n confirming:101
LEAP-Seq n nonconfirming:232
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GGTCAACGCAGACCGTTTAT
Suggested primer 2:AAAAACCGTCTGCACCTGTC