| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.108167 |
| Chromosome: | chromosome 8 |
| Location: | 4580932 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g383050 | (1 of 1) 3.7.1.14 - 2-hydroxy-6-oxonona-2,4-dienedioate hydrolase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGGCTAACCATCAAGTCCTCTATCTCAC |
| Internal bar code: | GGAGAAATGAGGAGGCGGCGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 810 |
| LEAP-Seq percent confirming: | 99.0965 |
| LEAP-Seq n confirming: | 5594 |
| LEAP-Seq n nonconfirming: | 51 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATCACGTCACGCCATACAC |
| Suggested primer 2: | CGGCTTGTTTTGGTGGTAGT |