Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.108191 |
Chromosome: | chromosome 5 |
Location: | 1639803 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre05.g243150 | Carbonic anhydrase-related protein; (1 of 1) PF04357 - Family of unknown function (DUF490) (DUF490) | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTTGTATAACAAAATGGCCACACGGATC |
Internal bar code: | ACATGTCATTGGAAACTATGGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 279 |
LEAP-Seq percent confirming: | 72.2864 |
LEAP-Seq n confirming: | 313 |
LEAP-Seq n nonconfirming: | 120 |
LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTTGGCCGTGTGTTAGGAAT |
Suggested primer 2: | CCAACACCAACACACACACA |