| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.108203 |
| Chromosome: | chromosome 11 |
| Location: | 834480 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467648 | RBL3 | Rhomboid-like protease; (1 of 4) PTHR22936//PTHR22936:SF32 - RHOMBOID-RELATED // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGCGATGCCCGAATTCGCGCGAGTTCGG |
| Internal bar code: | GGGGGCAGCAAGTCTAGGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 595 |
| LEAP-Seq percent confirming: | 98.4375 |
| LEAP-Seq n confirming: | 378 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACATTGTGCACGAAGAGCTG |
| Suggested primer 2: | GAGTTGTAAGGCAGTCCCCA |