Insertion junction: LMJ.RY0402.108206_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.108206
Chromosome:chromosome_5
Location:2080453
Confidence (%):73
Locus systematic id Locus common name Defline Orientation Feature
None

Insertion site details

Flanking sequence (orientation from cassette outwards):ACGCACGGACGCACACGCACACACCACCAC
Internal bar code:GGGCGCCTTCGAGCTGCTGGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:606
LEAP-Seq percent confirming:99.8081
LEAP-Seq n confirming:520
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:TGTCTCATCATCTTGCGCTC
Suggested primer 2:ACCGCTCATGCTTTGACTCT