Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.108206 |
Chromosome: | chromosome_5 |
Location: | 2117563 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre05.g234640 | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GACAGCGCCCTTCTAGCAAAGCCCGGTACG |
Internal bar code: | GTAGGGAGCGCTAGCAACACTG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 640 |
LEAP-Seq percent confirming: | 99.0021 |
LEAP-Seq n confirming: | 1885 |
LEAP-Seq n nonconfirming: | 19 |
LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CGGTTTAGTTTGGGCTGGTA |
Suggested primer 2: | CAAGAAGAGGTTCGGACAGC |