| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.108208 |
| Chromosome: | chromosome 2 |
| Location: | 4029221 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g097200 | SRR14 | Scavenger receptor cysteine rich (SRCR) protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCATGGAAATGGCGTAAACAAGGAAGTC |
| Internal bar code: | CTCGAGTGCCGTCCTTCCTGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 233 |
| LEAP-Seq percent confirming: | 96.0 |
| LEAP-Seq n confirming: | 72 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACTTCTCCTCCACCTGCTG |
| Suggested primer 2: | CGTGTCATCACGTGCTTCTT |