| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.108234 |
| Chromosome: | chromosome 13 |
| Location: | 251252 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g563733 | (1 of 1) K04368 - mitogen-activated protein kinase kinase 1 (MAP2K1, MEK1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGGCAGCGCGGGCGGCGGCTCCATCACAA |
| Internal bar code: | GCGTGCCATCTAGGATTCCACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 16 |
| LEAP-Seq percent confirming: | 90.3981 |
| LEAP-Seq n confirming: | 386 |
| LEAP-Seq n nonconfirming: | 41 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGGGCAAGAAGGTGGTTTT |
| Suggested primer 2: | GCTCTCCACTGGCACTAACC |